Spectroscopy White Papers and Case Studies

  1. Frictionless Precision Motion Control Based On Air Bearing Stages - Key Element In Coordinate Measuring And Surface Metrology Applications

    The performance of precision metrology equipment such as coordinate measuring machines (CMM) depends on highly accurate, repeatable, and controllable motion systems and position sensors. Air bearings have played a critical role in the development and construction of CMMs and other metrology tools. They are inherently frictionless, highly stable, and feature geometric precision straightness and flatness for linear bearings.

  2. Minimizing Trade-Offs Between Spectral Smoothing And Resolution

    Boxcar smoothing is a convenient way to smooth out noise in a spectrum, enabling peaks of interest to be more easily viewed, defined and characterized for properties like peak intensity, center wavelength, and FWHM (full width half maximum). It is a convenient complement to spectral averaging, particularly to minimize acquisition times, but must be used judiciously to avoid impacting the spectral resolution of the data acquired.

  3. Noise In Spectrometers: Part 2

    Noise is an undesirable signal that is completely lacking in information or structure, and potentially takes away from the desired signal’s quality and accuracy. Part one of this two-part series discussed the sources of noise from a spectrometer and its experimental methods. This second part of the series takes the information gleaned in part one and uses it to measure low concentrations of sports drinks in a pool, develop a calibration curve,  and determine the limit of detection for the spectrometer.

  4. Noise In Spectrometers: Part 1

    This application note is the first part in a series discussing the noise within spectroscopy systems used for ordinary applications, such as making sure the pool is clean after a large family gathering.

  5. CCD Cameras And Imaging Spectrographs Facilitate Acquisition Of Femtosecond Stimulated Raman Spectroscopy Data

    Characterizing surface-enhanced Raman spectroscopy (SERS) biosensors, or fluorescent dye molecules for in vivo bioanalyte detection, can be difficult due to the overwhelming isoenergetic fluorescence signal that makes it challenging to measure resonance Raman cross-sections for molecules. As a solution, etalon-based femtosecond stimulated Raman spectroscopy (FSRS) has been used to acquire a stimulated Raman signal without strong fluorescence or interference from signals. This article focuses on this approach to determining the resonance Raman cross-sections for use in biological SERS sensing with femtosecond stimulated Raman spectroscopy.

  6. Medical Research With FLIR Thermal Imaging Cameras

    Tissue autofluorescence spectroscopy, or the measurement of intrinsic light emissions from biological agents upon excitation, is utilized in a variety of biomedical research for differentiating between diseased and normal tissues. Researchers interested in the correlation between temperature and tissue autofluorescence spectroscopy during surgery are finding their answers with the help of thermal imaging. FLIR offers a scientific camera with the ideal resolution and lens magnification for such research. This application note offers the full story on how researchers used FLIR cameras and ResearchIR software to work to gain a greater understanding of this surgical tool.

  7. NIR Spectroscopy Unbound

    Near-infrared (NIR) spectroscopy covers the wavelength space between ultraviolet-visible and infrared absorbance. With enormous potential for a variety of applications, NIR spectroscopy is much more accessible due to the availability of compact, high-value NIR spectrometers. This application note provides a brief introduction to NIR spectroscopy, and introduces the breakthrough Flame-NIR spectrometer from Ocean Optics for handheld and portable applications requiring NIR and chemometric analysis. 

  8. NIR Spectroscopy Aids In The Diagnosis Of Neonatal Brain Injury

    An extensive set of spectroscopy-based methods have been developed over the past few years in order to facilitate more rapid and accurate detection and diagnosis of disease and injury. A British research group has recently designed and tested an NIRS system to assist in the diagnosis of neonatal brain injury. This new system measures the changes in tissue oxygenation and hemodynamics by estimating the changes in hemoglobin concentration. This application note presents a study on the diagnosis of neonatal brain injury through the use of CYRIL and quantitative near-infrared spectroscopy.

  9. More Raman Wavelengths, More Choices: The Utility Of Raman Spectroscopy

    Raman spectroscopy plays a huge part in the analysis of materials such as explosives in the field, pharmaceutical ingredients in the lab, and changes in genetic material, proteins, and lipids in clinical settings. Utilizing non-elastic scattering of laser light from a molecule to probe the molecular structure and create a vibrational transition in the sample, a molecular fingerprint is gradually built up. There are multiple wavelength analysis selections that can be used for a wide variety of applications. This application note discusses the benefits of using each wavelength, and presents different applications they are used for.

  10. Raman Spectroscopy Of Pigmented Human Tissue In The Shortwave Infrared

    The researchers at Erasmus MC University Medical Center in the Netherlands have developed a method of Raman spectroscopy that will substantially further and accelerate the diagnosis of melanoma. This app note discusses how technology developed by Xenics plays a key factor in this research.