Application Note

UV Raman Spectroscopy

Raman spectroscopy is a powerful analytical technique, but it faces challenges such as small Raman scattering cross sections and intrinsic noise sources like sample autofluorescence. Traditional Raman measurements use green, red, or near-infrared (IR) lasers. However, shifting to ultraviolet (UV) wavelengths can significantly mitigate these issues. UV lasers enhance the intensity of Raman-active vibrations through resonance-enhanced Raman scattering, offering a higher signal-to-noise ratio.

Despite strong autofluorescence excited by UV lasers, it does not interfere with the Raman signal when using common 266 nm lasers. The rise of compact, high-power UV lasers and advanced optical filters, such as Semrock's RazorEdge® and MaxLine® filters, has propelled UV Raman spectroscopy into more widespread use, enabling ultra-sensitive measurements and expanding its applications.

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