Microscopy White Papers and Case Studies

  1. Optical Components For SWIR Imaging
    7/8/2019

    This application note provides information on the benefits of SWIR imaging in harsh or low-light level conditions, and PG&O's capabilities in manufacturing optical components for SWIR imaging.

  2. Improving Microscopy And Biological Imaging With Backside Illuminated (BI) sCMOS Sensors
    5/20/2019

    Biological labs can get better and more quantifiable image data from microscopes with sCMOS sensor-based devices.

  3. DNA-PAINT: A Super-Resolution Microscopy Technique
    10/16/2018

    The recently introduced super-resolution method DNA-PAINT is based on transient DNA-DNA interactions. This white paper discusses the DNA-PAINT microscopy technique and how sCMOS cameras are well suited for these kinds of measurements.

  4. Xeva-1.7-320 Brochure: SWIR Imaging Camera for Advanced Research
    5/16/2018

    Xenics’ Xeva-1.7-320 digital camera combines a thermo-electrically cooled InGaAs detector head and the control and communication electronics in a compact housing. Single or three-stage Peltier cooling reduces the noise and dark current for enhanced low light-level imaging. The Xeva-1.7-320 combines standard (up to 1.7 µm) InGaAs detector arrays with various speed versions: 60 Hz, 100 Hz and 350 Hz. This allows you to choose the most suitable detector-camera configuration for applications including SWIR microscopy, hyperspectral imaging, laser beam profiling, low-light level imaging, semiconductor inspection, and medical applications.

  5. Micromirror TIRF Microscopy: Technique And Applications
    11/29/2017

    Total internal reflection fluorescence (TIRF) microscopy is used to watch biological processes unfold in real time. Taking advantage of the ability to label individual molecules with different colors of fluorescent tags, TIRF microscopy lets scientists view the complex molecular assemblies that govern cellular processes.

  6. Understanding Interference Tilted Excitation (LITE) Microscopy
    11/15/2017

    When the detection of a cellular or subcellular structure requires a high NA detection objective, conventional fluorescence microscopy and light sheet fluorescence microscopy (LSFM) encounter challenges. Lateral interference tilted excitation (LITE) microscopy overcomes this problem.

  7. Understanding Spinning Disk Confocal Microscopy
    11/15/2017

    Conventional fluorescence microscopy, used for biological imaging, does not construct 3-D images needed for 3-D specimens, and it is not able to gaze past cell features. Confocal microscopy uses optical sectioning to obtain multiple, thin, 2-demensional slices of a sample to construct 3-D models. 

  8. Understanding Light Sheet Microscopy
    11/15/2017

    Light sheet microscopy is a technique that images a mostly large sample with short time intervals under healthier conditions over a longer period of time than conventional types of microscopy. This white paper introduces the technology in brief, walks through sample preparation for the technique, and describes its advantages and drawbacks.

  9. Understanding Oblique Single Plane Illumination Microscopy (oSPIM)
    11/14/2017

    Oblique Single Plane Illumination Microscopy (oSPIM) is a platform for high resolution light sheet microscopy that combines the low photo-bleaching and photo-damage of LSFM with high magnification, high NA objectives for cellular and subcellular imaging. This paper describes how oSPIM works, its advantages over conventional fluorescence microscopy, and the equipment required for this technique.

  10. Automation And Microscopy: Faster Test And Measurement
    10/5/2017

    Nanotechnology uses the concept that constant velocity will get you to the destination faster than constantly stopped traffic. One technique puts this concept into practice via a fast nano-focus device based on a piezo-ceramic actuator embedded in a flexure guided lens positioning mechanism.