Microscopy White Papers and Case Studies

  1. Xeva-1.7-320 Brochure: SWIR Imaging Camera for Advanced Research
    5/16/2018

    Xenics’ Xeva-1.7-320 digital camera combines a thermo-electrically cooled InGaAs detector head and the control and communication electronics in a compact housing. Single or three-stage Peltier cooling reduces the noise and dark current for enhanced low light-level imaging. The Xeva-1.7-320 combines standard (up to 1.7 µm) InGaAs detector arrays with various speed versions: 60 Hz, 100 Hz and 350 Hz. This allows you to choose the most suitable detector-camera configuration for applications including SWIR microscopy, hyperspectral imaging, laser beam profiling, low-light level imaging, semiconductor inspection, and medical applications.

  2. Micromirror TIRF Microscopy: Technique And Applications
    11/29/2017

    Total internal reflection fluorescence (TIRF) microscopy is used to watch biological processes unfold in real time. Taking advantage of the ability to label individual molecules with different colors of fluorescent tags, TIRF microscopy lets scientists view the complex molecular assemblies that govern cellular processes.

  3. Understanding Interference Tilted Excitation (LITE) Microscopy
    11/15/2017

    When the detection of a cellular or subcellular structure requires a high NA detection objective, conventional fluorescence microscopy and light sheet fluorescence microscopy (LSFM) encounter challenges. Lateral interference tilted excitation (LITE) microscopy overcomes this problem.

  4. Understanding Spinning Disk Confocal Microscopy
    11/15/2017

    Conventional fluorescence microscopy, used for biological imaging, does not construct 3-D images needed for 3-D specimens, and it is not able to gaze past cell features. Confocal microscopy uses optical sectioning to obtain multiple, thin, 2-demensional slices of a sample to construct 3-D models. 

  5. Understanding Light Sheet Microscopy
    11/15/2017

    Light sheet microscopy is a technique that images a mostly large sample with short time intervals under healthier conditions over a longer period of time than conventional types of microscopy. This white paper introduces the technology in brief, walks through sample preparation for the technique, and describes its advantages and drawbacks.

  6. Understanding Oblique Single Plane Illumination Microscopy (oSPIM)
    11/14/2017

    Oblique Single Plane Illumination Microscopy (oSPIM) is a platform for high resolution light sheet microscopy that combines the low photo-bleaching and photo-damage of LSFM with high magnification, high NA objectives for cellular and subcellular imaging. This paper describes how oSPIM works, its advantages over conventional fluorescence microscopy, and the equipment required for this technique.

  7. Automation And Microscopy: Faster Test And Measurement
    10/5/2017

    Nanotechnology uses the concept that constant velocity will get you to the destination faster than constantly stopped traffic. One technique puts this concept into practice via a fast nano-focus device based on a piezo-ceramic actuator embedded in a flexure guided lens positioning mechanism.

  8. When Custom Costs Less Than Standard: The Benefits And Flexibility Of Custom Optical Assemblies
    8/24/2017

    Here are a few questions you might be asking when you’re considering whether to build your own assembly or have it custom-made by an experienced design and manufacturing team.

  9. Confocal Microscopy Evolves With AO Tunable Filters
    10/13/2016

    Acousto-optical tunable filter (AOTF) technology is growing to replace conventional and cumbersome mechanical techniques within biomedical applications for confocal microscopy. Benefits of using AO tunable systems include electronic control, configurable drivers for improving operator flexibility, feedback stabilizing systems to maintain wavelength stability for all environmental conditions, and custom-grown tellurium dioxide crystals to ensure optimal performance. This article discusses the evolution of confocal microscopy in using AOTF technologies, as well as maximizing the potential of AOTF systems and their benefits to users by integrating next-generation drivers.

  10. Comparison Of Scientific Cameras For Fluorescence Microscopy
    9/14/2016

    Raptor Photonics recently compared the performance of four of their scientific cameras in fluorescence microscopy. Each camera utilized a different sensor technology, EMCCD, sCMOS and CCD under similar conditions in order to find the advantages of each one.