Total internal reflection microscopy (TIRFM) is an optical technique used to observe single molecule fluorescence. Some biophysicists have used the technique for many years, while others are just beginning to explore the boundaries of this versatile mechanism for studying phenomena occurring at interfaces. A recently developed high numerical aperture microscope objective lens that improves TIRFM and makes it widely accessible is discussed in this application note
Transverse wave light whose vibration possess direction is called polarized light. Light from an ordinary light source (natural light) that vibrates in random directions is called nonpolarized light
