Microscopy White Papers and Case Studies

  1. Application Note: Nonlinear Microscopy
    Ultrafast lasers have revolutionized the field of microscopy by enabling new types of nonlinear microscopy techniques. Femtosecond pulse lasers provide the combination of high peak power (1-100 kW) and low average power (10-1000mW) which is ideal for utilizing nonlinear optical processes in scanning laser microscopy (LSM). Here, we describe the two main varieties of nonlinear microscopy: two-photon microscopy, and third-harmonic generation (THG) microscopy. And we show images using these techniques with various types of modelocked fiber lasers. By IMRA America, Inc.
  2. White Paper: Optimization Of Spinning Disk Confocal Microscopy: Synchronization With The Ultra-Sensitive EMCCD
    There has been a tremendous growth in the popularity of confocal microscopy in recent years, due in part to the relative ease with which extremely high-quality images can be obtained from specimens prepared for conventional optical microscopy particularly for live cell imaging
  3. Technical Note: Luca-R EMCCD vs. Interline CCD For Cell Microscopy Applications
    In this study we compared the sensitivity and resolution performance of the Luca-R against the highest specification interline CCD on the market, in a cell microscopy application
  4. Application Note: Total Internal Reflection Fluorescence Microscopy
    Total internal reflection microscopy (TIRFM) is an optical technique used to observe single molecule fluorescence. Some biophysicists have used the technique for many years, while others are just beginning to explore the boundaries of this versatile mechanism for studying phenomena occurring at interfaces. A recently developed high numerical aperture microscope objective lens that improves TIRFM and makes it widely accessible is discussed in this application note
  5. White Paper: Basics Of Polarizing Microscopy
    Transverse wave light whose vibration possess direction is called polarized light. Light from an ordinary light source (natural light) that vibrates in random directions is called nonpolarized light