This brochure includes information on PI’s offerings as they relate to microscopy applications.
PI offers Piezo and motion control solutions for microdosing, biohandling, computer aided surgery, microscopy, and other medical applications.
The field of biological imaging is constantly evolving, driven forward by the increasingly complex and technologically sophisticated demands of the research community. As microscopy and other imaging techniques change, so too must the motion control systems that enable them. Photonics Online recently had the opportunity to talk with John Zemek, executive director of Applied Scientific Instrumentation. ASI manufactures sub-micron micropositioning hardware for laboratory and microscope automation and OEM systems. Zemek discusses the trends in biological imaging that are having the biggest influence on positioning system design, and what factors you should consider when evaluating such technology for your specific application.
Cameras and scanning systems used in applications involving fluorescence microscopy, white-light interferometry OCT, or aerial photography need to be high resolution. When resolution needs to be increased, there are only a few options
When scientific CMOS (sCMOS) image sensors debuted in 2009 at the Laser World of Photonics meeting, the imaging world knew the technology could be big. Three years later, sCMOS image sensors and their uses continue to grow. By Gerhard Holst, Head of the Science & Research Department, PCO AG
It has been established that optical techniques based on nonlinear processes, such as two‐photon excited fluorescence (TPEF) and second harmonic generation (SHG), are advantageous for microscopic imaging at depth [1‐4]. When compared with confocal or wide‐field microscopy techniques, they provide greater penetration depths and superior image contrast. The nonlinear optical response to a focused laser field is intrinsically confined to the focal volume, which helps mitigating light scattering effects and allows imaging at depths as large as four‐five mean free path lengths [5‐7]. By Biophotonic Solutions Inc.
Superresolution Microscopy is a technique used to achieve an image resolution beyond the diffraction limit that is common in conventional light microscopes. This whitepaper discusses the superresolution structured illumination method and how it is used to achieve three dimensional resolution enhancement through a combination of optics and image analysis.
STED (Stimulated Emission Depletion) Microscopy is a technique used to overcome light diffraction and spatial resolution images encountered in traditional optical microscopy. STED Microscopy goes beyond the spatial diffraction-limit, allowing for clear image capture of the fabric of living cells. STED Microscopy, along with GSD (Ground-State Depletion) Microscopy, are also known as fluorescence nanoscopy. This application note presents the results of an experiment conducted through the use of a Cobolt Flamenco™ 660 nm continuous wave single frequency DPSS laser in a STED nanoscopy setup.
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