Microscopy White Papers and Case Studies

31. Piezo Mechanisms In Optics And Laser Technology 7/10/2013

    Laser technology is crucial for the advancement of many high tech fields from aerospace to medical diagnostics. By Stefan Vorndran, Scott Jordan, Steffen Arnold, PI (Physik Instrumente)

32. An Introduction To Digital Microscopy Imaging 5/30/2013

    As fun as taking pictures may be, it is the professional analysis of samples that really counts. This article discusses key concepts of digital microscopy imaging systems and how these concepts contribute to a professional presentation of your work. By SPOT Imaging Solutions, a division of Diagnostic Instruments, Inc.

33. PI Products For Microscopy 5/28/2013

    This brochure includes information on PI’s offerings as they relate to microscopy applications.

34. Piezo and Motion Control Solutions for Medical Applications 5/28/2013

    PI offers Piezo and motion control solutions for microdosing, biohandling, computer aided surgery, microscopy, and other medical applications.

35. 3 Trends In Biological Imaging — And Their Impact On Microscope Automation And Control 5/17/2013

    The field of biological imaging is constantly evolving, driven forward by the increasingly complex and technologically sophisticated demands of the research community. As microscopy and other imaging techniques change, so too must the motion control systems that enable them. Photonics Online recently had the opportunity to talk with John Zemek, executive director of Applied Scientific Instrumentation. ASI manufactures sub-micron micropositioning hardware for laboratory and microscope automation and OEM systems. Zemek discusses the trends in biological imaging that are having the biggest influence on positioning system design, and what factors you should consider when evaluating such technology for your specific application.

36. Resolution Enhancement of Imaging Chips with Piezo Technology 3/15/2013

    Cameras and scanning systems used in applications involving fluorescence microscopy, white-light interferometry OCT, or aerial photography need to be high resolution. When resolution needs to be increased, there are only a few options

37. Pulse Compression For Ultrafast Nonlinear Microscopy 8/20/2012

    It has been established that optical techniques based on nonlinear processes, such as two‐photon excited fluorescence (TPEF) and second harmonic generation (SHG), are advantageous for microscopic imaging at depth [1‐4]. When compared with confocal or wide‐field microscopy techniques, they provide greater penetration depths and superior image contrast. The nonlinear optical response to a focused laser field is intrinsically confined to the focal volume, which helps mitigating light scattering effects and allows imaging at depths as large as four‐five mean free path lengths [5‐7]. By Biophotonic Solutions Inc.

38. Superresolution Structured Illumination Microscopy (SR-SIM) 6/15/2012

    Superresolution Microscopy is a technique used to achieve an image resolution beyond the diffraction limit that is common in conventional light microscopes. This whitepaper discusses the superresolution structured illumination method and how it is used to achieve three dimensional resolution enhancement through a combination of optics and image analysis. 

39. Cobolt Flamenco 660 nm for Stimulated Emission Depletion (STED) Microscopy 6/4/2012

    STED (Stimulated Emission Depletion) Microscopy is a technique used to overcome light diffraction and spatial resolution images encountered in traditional optical microscopy. STED Microscopy goes beyond the spatial diffraction-limit, allowing for clear image capture of the fabric of living cells. STED Microscopy, along with GSD (Ground-State Depletion) Microscopy, are also known as fluorescence nanoscopy. This application note presents the results of an experiment conducted through the use of a Cobolt Flamenco™ 660 nm continuous wave single frequency DPSS laser in a STED nanoscopy setup.

40. Application Note: CARS Microscopy 8/10/2010
    In the last ten years multi-photon-microscopy has become increasingly popular to enhancing contrast and resolution for modern microscopic imaging due to the utilization of non-linear optical effects. Amongst many methods of multi-photon-microscopy, two-photon-excitation fluorescence- microscopy is the most popular one with more than a few hundred systems installed every year. The big draw back of two-photon-fluorescence microscopy, however, is the use of dyes for creating the images. Methods to overcome the problems connected with dyes are based on non-linear optical effects such as second and third harmonic generation (SHG, THG), Raman scattering or Coherent Antistokes Raman Scattering (CARS), a third order non-linear process (X(3) process) involving four photons.