Microscopy White Papers and Case Studies

  1. When Custom Costs Less Than Standard: The Benefits And Flexibility Of Custom Optical Assemblies

    Optical subsystems exist in a wide variety of configurations for a whole range of applications. Most of the parts that go into such optomechanical assemblies — laser and LED light sources, lenses, dichroics, filaments, detectors, spatial light modulators, and all the associated mechanical fixtures — are available as commercial off-the-shelf (COTS) parts. So it can be tempting to say that if you’re designing a new optical subsystem, you should just purchase the parts and build it yourself. But is that really the right choice? Here are a few questions you might be asking when you’re considering whether to build your own assembly or have it custom-made by an experienced design and manufacturing team.

  2. Automation And Microscopy: Faster Test And Measurement

    Nanotechnology uses the concept that constant velocity will get you to the destination faster than constantly stopped traffic. One technique puts this concept into practice via a fast nano-focus device based on a piezo-ceramic actuator embedded in a flexure guided lens positioning mechanism.

  3. Confocal Microscopy Evolves With AO Tunable Filters

    Acousto-optical tunable filter (AOTF) technology is growing to replace conventional and cumbersome mechanical techniques within biomedical applications for confocal microscopy. Benefits of using AO tunable systems include electronic control, configurable drivers for improving operator flexibility, feedback stabilizing systems to maintain wavelength stability for all environmental conditions, and custom-grown tellurium dioxide crystals to ensure optimal performance. This article discusses the evolution of confocal microscopy in using AOTF technologies, as well as maximizing the potential of AOTF systems and their benefits to users by integrating next-generation drivers.

  4. Comparison Of Scientific Cameras For Fluorescence Microscopy

    Raptor Photonics recently compared the performance of four of their scientific cameras in fluorescence microscopy. Each camera utilized a different sensor technology, EMCCD, sCMOS and CCD under similar conditions in order to find the advantages of each one.

  5. Air Bearing FAQs

    This blog article presents questions and answers on air bearing positioning equipment.

  6. Super-Resolution Optical Microscopy, Nanoscopy, And Nanopositioning Motion Control

    This article presents the new frontier for super-resolution optical microscopy, nanoscopy, and nanopositioning motion control.

  7. Compact, Lens-Free Microscope Demo Kit Adapts To User Applications

    Lens-free microscopy enables an abundance of applications to which traditional microscopes are less amenable. Examples include particle monitoring in a cleanroom environment or cell monitoring with the microscope integrated in the biotech tool. Built on earlier prototypes, imec’s lens-free digital microscope is offered as a ready-to-use evaluation kit for life sciences and industrial applications. This demo kit allows companies to try out their own applications and, with the help of imec’s engineers, fine-tune the tool toward their own requirements.

  8. Optical Components for SWIR Imaging

    This application note provides information on the benefits of SWIR imaging in harsh or low-light level conditions, and PG&O's capabilities in manufacturing optical components for SWIR imaging.

  9. Using Next-Generation Thin-Film Optical Filters For Fluorescence Imaging And Detection Systems

    Within any fluorescence application, there are three basic filters that are needed for operation – the excitation filter, the emission filter, and the beamsplitter. Hard-coated, thin-film optical filters are the best choice for these filters in that they provide the highest standards such as improvements in transmission, blocking, and both transmitted and reflected wavefront properties, which translates into bright, high-contrast images, and accurate detection of target molecules in fluorescence applications. This white paper further discusses the use of Alluxa’s thin-film optical filters in fluorescence imaging and detection systems.

  10. Interference Microscopy For Surface Structure Analysis

    Interference microscopy encompasses a wide range of techniques when it comes to measuring the many aspects of surface structures. Utilizing interference microscopy for these types of measurements includes the advantages of rapid, non-contact operation and rapid data acquisition, often at data acquisition rates in the millions of 3D image points per second. Due to its uniqueness among optical areal measurement instruments, interferometry has the benefit of high sensitivity to surface structures. This article discusses the principles of interferometric dimensional metrology applied to surface features best viewed in a microscope