Microscopy White Papers and Case Studies

  1. Understanding Interference Tilted Excitation (LITE) Microscopy

    When the detection of a cellular or subcellular structure requires a high NA detection objective for the superior resolution and light collection efficiency, conventional fluorescence microscopy and Light Sheet Fluorescence Microscopy (LSFM) encounter challenges. Imaging these structures is difficult and could only be achieved through multi-view SPIM geometries with a 1.2 NA objective and subsequent deconvolution – an incredibly time consuming and data heavy process. Lateral Interference Tilted Excitation (LITE) microscopy overcomes this problem by eliminating the illumination objective and introducing a tilted light sheet through a photomask and cylindrical lens that can converge at the working distance of high NA objectives.

  2. Understanding Spinning Disk Confocal Microscopy

    Using conventional fluorescence microscopy for biological imaging can be challenging because this technique does not construct 3-D images needed for 3-D specimens, and it is not able to gaze past cell features to view what happens inside certain biological structures. Confocal microscopy uses optical sectioning to obtain multiple, thin, 2-demensional slices of a sample to construct 3-D models. This enables studying 3-D structures with fast dynamic processes, long-term time-lapses, or details inside the cell membrane, all possible with live cells.

  3. Understanding Light Sheet Microscopy

    Microscopy technologies are continuously striving to exploit new boundaries by aiming for smaller details, and becoming more sensitive and less noisy. Light sheet microscopy, first discovered by Richard Zsigmondy and Henry Siedentopf in 1903, is a technique that images a mostly large sample with short time intervals under healthier conditions over a longer period of time than conventional types of microscopy. This white paper introduces the technology in brief, walks through sample preparation for the technique, and describes its advantages and drawbacks.

  4. Understanding Oblique Single Plane Illumination Microscopy (oSPIM)

    Oblique Single Plane Illumination Microscopy (oSPIM) is a platform for high resolution light sheet microscopy that combines the low photo-bleaching and photo-damage of LSFM with high magnification, high NA objectives for cellular and subcellular imaging. This paper describes how oSPIM works, its advantages over conventional fluorescence microscopy, and the equipment required for this technique.

  5. Automation And Microscopy: Faster Test And Measurement

    Nanotechnology uses the concept that constant velocity will get you to the destination faster than constantly stopped traffic. One technique puts this concept into practice via a fast nano-focus device based on a piezo-ceramic actuator embedded in a flexure guided lens positioning mechanism.

  6. When Custom Costs Less Than Standard: The Benefits And Flexibility Of Custom Optical Assemblies

    Optical subsystems exist in a wide variety of configurations for a whole range of applications. Most of the parts that go into such optomechanical assemblies — laser and LED light sources, lenses, dichroics, filaments, detectors, spatial light modulators, and all the associated mechanical fixtures — are available as commercial off-the-shelf (COTS) parts. So it can be tempting to say that if you’re designing a new optical subsystem, you should just purchase the parts and build it yourself. But is that really the right choice? Here are a few questions you might be asking when you’re considering whether to build your own assembly or have it custom-made by an experienced design and manufacturing team.

  7. Confocal Microscopy Evolves With AO Tunable Filters

    Acousto-optical tunable filter (AOTF) technology is growing to replace conventional and cumbersome mechanical techniques within biomedical applications for confocal microscopy. Benefits of using AO tunable systems include electronic control, configurable drivers for improving operator flexibility, feedback stabilizing systems to maintain wavelength stability for all environmental conditions, and custom-grown tellurium dioxide crystals to ensure optimal performance. This article discusses the evolution of confocal microscopy in using AOTF technologies, as well as maximizing the potential of AOTF systems and their benefits to users by integrating next-generation drivers.

  8. Comparison Of Scientific Cameras For Fluorescence Microscopy

    Raptor Photonics recently compared the performance of four of their scientific cameras in fluorescence microscopy. Each camera utilized a different sensor technology, EMCCD, sCMOS and CCD under similar conditions in order to find the advantages of each one.

  9. Air Bearing FAQs

    This blog article presents questions and answers on air bearing positioning equipment.

  10. Super-Resolution Optical Microscopy, Nanoscopy, And Nanopositioning Motion Control

    This article presents the new frontier for super-resolution optical microscopy, nanoscopy, and nanopositioning motion control.