Microscopy White Papers and Case Studies

  1. Understanding Spinning Disk Confocal Microscopy 11/15/2017

    Conventional fluorescence microscopy, used for biological imaging, does not construct 3-D images needed for 3-D specimens, and it is not able to gaze past cell features. Confocal microscopy uses optical sectioning to obtain multiple, thin, 2-demensional slices of a sample to construct 3-D models. 

  2. Understanding Light Sheet Microscopy 11/15/2017

    Light sheet microscopy is a technique that images a mostly large sample with short time intervals under healthier conditions over a longer period of time than conventional types of microscopy. This white paper introduces the technology in brief, walks through sample preparation for the technique, and describes its advantages and drawbacks.

  3. Understanding Oblique Single Plane Illumination Microscopy (oSPIM) 11/14/2017

    Oblique Single Plane Illumination Microscopy (oSPIM) is a platform for high resolution light sheet microscopy that combines the low photo-bleaching and photo-damage of LSFM with high magnification, high NA objectives for cellular and subcellular imaging. This paper describes how oSPIM works, its advantages over conventional fluorescence microscopy, and the equipment required for this technique.

  4. Automation And Microscopy: Faster Test And Measurement 10/5/2017

    Nanotechnology uses the concept that constant velocity will get you to the destination faster than constantly stopped traffic. One technique puts this concept into practice via a fast nano-focus device based on a piezo-ceramic actuator embedded in a flexure guided lens positioning mechanism.

  5. When Custom Costs Less Than Standard: The Benefits And Flexibility Of Custom Optical Assemblies 8/24/2017

    Here are a few questions you might be asking when you’re considering whether to build your own assembly or have it custom-made by an experienced design and manufacturing team.

  6. Confocal Microscopy Evolves With Acousto-Optical Tunable Filters (AOTF) 10/13/2016

    Acousto-optical tunable filter (AOTF) technology is replacing conventional mechanical techniques within biomedical applications for confocal microscopy. This article discusses the evolution of confocal microscopy in using AOTF technologies, as well as maximizing the potential of AOTF systems and their benefits.

  7. Air Bearing FAQs 8/5/2016

    This blog article presents questions and answers on air bearing positioning equipment.

  8. Super-Resolution Optical Microscopy, Nanoscopy, And Nanopositioning Motion Control 8/5/2016

    This article presents the new frontier for super-resolution optical microscopy, nanoscopy, and nanopositioning motion control.

  9. Compact, Lens-Free Microscope Demo Kit Adapts To User Applications 7/8/2016

    Lens-free microscopy enables an abundance of applications to which traditional microscopes are less amenable. Examples include particle monitoring in a cleanroom environment or cell monitoring with the microscope integrated in the biotech tool. Built on earlier prototypes, imec’s lens-free digital microscope is offered as a ready-to-use evaluation kit for life sciences and industrial applications. This demo kit allows companies to try out their own applications and, with the help of imec’s engineers, fine-tune the tool toward their own requirements.

  10. Using Next-Generation Thin-Film Optical Filters For Fluorescence Imaging And Detection Systems 5/23/2016

    Within any fluorescence application, there are three basic filters that are needed for operation – the excitation filter, the emission filter, and the beamsplitter. This white paper further discusses the use of Alluxa’s thin-film optical filters in fluorescence imaging and detection systems.