Understanding Light Sheet Microscopy

By Photometrics

By Mathias Pasche and Dan Croucher, Photometrics

Microscopy technologies are continuously striving to exploit new boundaries by aiming for smaller details, and becoming more sensitive and less noisy. Light sheet microscopy, first discovered by Richard Zsigmondy and Henry Siedentopf in 1903, is a technique that images a mostly large sample with short time intervals under healthier conditions over a longer period of time than conventional types of microscopy. This white paper introduces the technology in brief, walks through sample preparation for the technique, and describes its advantages and drawbacks. Download the full paper for more in-depth information.