Application Note

Understanding Spinning Disk Confocal Microscopy

Source: Photometrics

Understanding Spinning Disk Confocal Microscopy

By Louis Keal and Dan Croucher, Photometrics

Using conventional fluorescence microscopy for biological imaging can be challenging because this technique does not construct 3-D images needed for 3-D specimens, and it is not able to gaze past cell features to view what happens inside certain biological structures. Confocal microscopy uses optical sectioning to obtain multiple, thin, 2-demensional slices of a sample to construct 3-D models. This enables studying 3-D structures with fast dynamic processes, long-term time-lapses, or details inside the cell membrane, all possible with live cells. Download the full paper for more in-depth information on the spinning disk confocal microscopy technique, as well as its advantages and drawbacks.