Technical Articles

  1. Understanding Interference Tilted Excitation (LITE) Microscopy

    When the detection of a cellular or subcellular structure requires a high NA detection objective for the superior resolution and light collection efficiency, conventional fluorescence microscopy and Light Sheet Fluorescence Microscopy (LSFM) encounter challenges. Lateral Interference Tilted Excitation (LITE) microscopy overcomes this problem by eliminating the illumination objective and introducing a tilted light sheet through a photomask and cylindrical lens that can converge at the working distance of high NA objectives.

  2. Understanding Spinning Disk Confocal Microscopy

    Conventional fluorescence microscopy, used for biological imaging, does not construct 3-D images needed for 3-D specimens, and it is not able to gaze past cell features. Confocal microscopy uses optical sectioning to obtain multiple, thin, 2-demensional slices of a sample to construct 3-D models. 

  3. Understanding Light Sheet Microscopy

    Microscopy technologies are continuously striving to exploit new boundaries by aiming for smaller details, and becoming more sensitive and less noisy. Light sheet microscopy, first discovered by Richard Zsigmondy and Henry Siedentopf in 1903, is a technique that images a mostly large sample with short time intervals under healthier conditions over a longer period of time than conventional types of microscopy. This white paper introduces the technology in brief, walks through sample preparation for the technique, and describes its advantages and drawbacks.

  4. Understanding Oblique Single Plane Illumination Microscopy (oSPIM)

    Oblique Single Plane Illumination Microscopy (oSPIM) is a platform for high resolution light sheet microscopy that combines the low photo-bleaching and photo-damage of LSFM with high magnification, high NA objectives for cellular and subcellular imaging. This paper describes how oSPIM works, its advantages over conventional fluorescence microscopy, and the equipment required for this technique.

  5. Factors To Consider When Purchasing A High-Speed Video Camera

    There are many factors that are important to consider when you purchase a high-speed camera. Photron USA, Inc. discusses the important factors involved in selecting the appropriate high-speed video camera for your specific information.

  6. A Q&A With Dr. Brian Pogue, Incoming Editor-in-Chief, SPIE Journal of Biomedical Optics

    Dr. Brian Pogue of Dartmouth College will take the helm as editor-in-chief of SPIE’s Journal of Biomedical Optics (JBO) starting Jan. 1, 2018. Pogue spoke with Photonics Online about his decision to join the JBO as editor-in-chief, his vision for the journal, and how he balances all of his interests in limited time.

  7. Benchtop Thermal Imaging: A Simple-To-Use, Cost-Effective Solution For Troubleshooting Complex Printed Circuit Boards

    Electronic products used in aerospace and other research applications have two key requirements: high performance and the reliability to withstand intense environments. To attain that performance, electronics designers often use small parts and routinely push them to their limits. 

  8. Creating A Passive Laser Beam Analyzer Through A Haas Laser Technologies And Xenics Partnership

    High power CO2 lasers produce a beam of infrared light with wavelengths centering typically at 9.3 or 10.6 μm. Haas Laser Technologies, Inc. has developed a compact, all passive optical design laser beam analyzer for focused laser beams from a CO2 laser with wavelength range from 9.3 to 10.6 μm. In conjunction with a Fabry-Perot resonator and Xenics’ Gobi-640-GigE microbolometer camera, the new beam analyzer offers laser beam measurement and analysis for continuous-wave (CW) as well as pulsed lasers.

  9. Seeing The Light: Sizing Up Universal Light Guides For Medical Instruments

    The Universal Light Guide is a workhorse of medical diagnostics that enables the high performance of high-end medical equipment such as endoscopes, the instruments of discovery that enable physicians to see, detect, diagnose and operate on parts of the body that are usually shielded from light.

  10. Automation And Microscopy: Faster Test And Measurement

    Nanotechnology uses the concept that constant velocity will get you to the destination faster than constantly stopped traffic. One technique puts this concept into practice via a fast nano-focus device based on a piezo-ceramic actuator embedded in a flexure guided lens positioning mechanism.