Technical Articles

  1. How To Make A Good Reflected Color Measurement

    When measuring reflection from an object, it is important to consider which type of reflection is dominant, and whether some or all the reflected light needs to be collected. This article discusses the best practices for making good reflected color measurements that can be used in a wide range of optical imaging applications.

  2. Thin-Film Optical Components For Use In Non-Linear Optical Systems

    Bio-imaging and detection techniques that use non-linear optical (NLO) phenomena have led to great advancements, such as super-resolution images, label-free visualization of naturally occurring biomolecules, and greater freedom for working with in-vivo samples. Many NLO systems rely on the high peak pulse intensity of femtosecond lasers for signal generation, and demand that optical filters and mirrors integrated into these systems have an appropriate laser damage rating, and the reflective components be controlled for both group delay dispersion (GDD) and flatness. This white paper discusses the importance of choosing thin-film optical components for NLO systems to ensure optimal signal strength, resolution, and image quality.

  3. Thin-Film Interference Filters For LIDAR

    LIDAR is now being used for obstacle avoidance in autonomous vehicles, urban planning, security, infrastructure development, and many other growing applications. This application note details how high-performance, ultra-narrowband interference filters improve LIDAR signal-to-noise ratios in specific applications.

  4. Understanding Interference Tilted Excitation (LITE) Microscopy

    When the detection of a cellular or subcellular structure requires a high NA detection objective for the superior resolution and light collection efficiency, conventional fluorescence microscopy and Light Sheet Fluorescence Microscopy (LSFM) encounter challenges. Imaging these structures is difficult and could only be achieved through multi-view SPIM geometries with a 1.2 NA objective and subsequent deconvolution – an incredibly time consuming and data heavy process. Lateral Interference Tilted Excitation (LITE) microscopy overcomes this problem by eliminating the illumination objective and introducing a tilted light sheet through a photomask and cylindrical lens that can converge at the working distance of high NA objectives.

  5. Understanding Spinning Disk Confocal Microscopy

    Using conventional fluorescence microscopy for biological imaging can be challenging because this technique does not construct 3-D images needed for 3-D specimens, and it is not able to gaze past cell features to view what happens inside certain biological structures. Confocal microscopy uses optical sectioning to obtain multiple, thin, 2-demensional slices of a sample to construct 3-D models. This enables studying 3-D structures with fast dynamic processes, long-term time-lapses, or details inside the cell membrane, all possible with live cells.

  6. Understanding Light Sheet Microscopy

    Microscopy technologies are continuously striving to exploit new boundaries by aiming for smaller details, and becoming more sensitive and less noisy. Light sheet microscopy, first discovered by Richard Zsigmondy and Henry Siedentopf in 1903, is a technique that images a mostly large sample with short time intervals under healthier conditions over a longer period of time than conventional types of microscopy. This white paper introduces the technology in brief, walks through sample preparation for the technique, and describes its advantages and drawbacks.

  7. Understanding Oblique Single Plane Illumination Microscopy (oSPIM)

    Oblique Single Plane Illumination Microscopy (oSPIM) is a platform for high resolution light sheet microscopy that combines the low photo-bleaching and photo-damage of LSFM with high magnification, high NA objectives for cellular and subcellular imaging. This paper describes how oSPIM works, its advantages over conventional fluorescence microscopy, and the equipment required for this technique.

  8. Factors To Consider When Purchasing A High-Speed Video Camera

    There are many factors that are important to consider when you purchase a high-speed camera. Photron USA, Inc. discusses the important factors involved in selecting the appropriate high-speed video camera for your specific information.

  9. A Q&A With Dr. Brian Pogue, Incoming Editor-in-Chief, SPIE Journal of Biomedical Optics

    Dr. Brian Pogue of Dartmouth College will take the helm as editor-in-chief of SPIE’s Journal of Biomedical Optics (JBO) starting Jan. 1, 2018. Pogue spoke with Photonics Online about his decision to join the JBO as editor-in-chief, his vision for the journal, and how he balances all of his interests in limited time.

  10. Benchtop Thermal Imaging: A Simple-To-Use, Cost-Effective Solution For Troubleshooting Complex Printed Circuit Boards

    Electronic products used in aerospace applications and for scientific research have two key requirements: they must have both the high performance and the reliability to withstand intense environments. To attain that performance, electronics designers often use small parts and routinely push them to their limits. But that means the parts get hot. If they get too hot, they can’t be counted on to work reliably. Sometimes, they even break.