Selecting Filters For Fluorescence Multiplexing

Fluorescence multiplexing, facilitated by advances in optical thin film deposition technology, has revolutionized the study of cellular components and signals by enabling the simultaneous use of multiple fluorophores in a single experiment.

Despite the availability of a diverse array of catalog filters designed for various fluorophores, selecting suitable filters remains a complex task. This white paper explores key considerations essential to the design of a robust multiplexing system that balances optimal optical performance with the demands of experimental flexibility and efficiency.