Application Note

Photodiode Detection In Veterinary CBC And Blood Cell Analysis

AN-09 Cover image

Break down the four optical measurements behind a complete blood count and matches a specific detector type to each one.

A veterinary hematology analyzer sorts millions of cells from a few microliters of blood in seconds, using forward scatter to size cells, side scatter to gauge internal complexity, fluorescence to flag reticulocytes and white-cell subsets, and absorbance to read hemoglobin. The core design principle is to treat these as four separate detector problems rather than specifying one general-purpose diode across the whole bench: a large-area, linear silicon photodiode for the bright forward-scatter signal; a low-dark-current silicon PIN photodiode (or a silicon avalanche photodiode where signal is scarce) for dim side-scatter and fluorescence; and a blue-enhanced silicon detector tuned to 540 nm for hemoglobin photometry.

The added difficulty in veterinary instruments is species variability—feline, canine, equine, and exotic blood all have different red-cell and platelet sizes—so the optical front end has to hold its resolution across species without re-tuning between patients.

The content includes a selection framework organized around laser wavelength and channel type, a comparison table of generic versus field-engineered detectors, a table of common failure modes (bright-channel saturation, weak-signal miss, hemoglobin drift, channel mismatch) with corresponding design responses, and a product table mapping six detector families to CBC channels like forward/side scatter, reticulocyte counting, and hemoglobin photometry.

It closes with custom engineering program details and contact information for the applications engineering team.

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