Application Note

Introduction To Next-Generation Thin-Film Optical Filters For Life Sciences

Source: Alluxa, Inc.

Fluorescence based systems have revolutionized the way organisms, cells, and biomolecules are visualized and detected. However, challenges that are common in these instruments, such as bleed-through, background autofluorescence, and poor signal-to-noise ratios (S/N), can reduce performance and lead to frustration. Because system performance greatly depends on filter quality, optical filters are arguably the most important component of any fluorescence-based instrument. With that in mind, this application note presents important concepts that should be considered when selecting optical filters for a fluorescence-based system.

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