How Do You Properly Orient Your Filters? Here's Your Guide

Semrock filters are renowned for their durability, enabling users to confidently assemble filter cubes, sliders, and wheels without risking damage. However, achieving optimal performance requires precise orientation to ensure intended transmission, blocking, and minimal autofluorescence. This article serves as a guide for correctly orienting different types of Semrock filters.

We provide detailed instructions on how to orient housed excitation and emission filters, ensuring alignment for optimal performance in microscopes. You'll also uncover the mystery behind orienting dichroic beamsplitters and other unhoused optical filters, with clear guidance on identifying coated surfaces and avoiding potential pitfalls. This guide will empower you to harness the full potential of Semrock filters for your fluorescence imaging needs.