Application Note

Flow Cytometry Filters With High-Performance Bandpass Optical Technology For Maximum Signal Clarity

Maximize spectral performance with Semrock Nanopede™ bandpass optical filters, engineered for flow cytometry and other precise optical applications. These filters deliver consistent, reliable results across the UV, visible, and NIR ranges while helping manage cost and complexity. The Nanopede™ family is designed to support spectral flow cytometry, where fluorescence from each cell is captured across the full spectrum. Two complementary flights of filters—Flight 1 (even 20 nm FWHM steps) and Flight 2 (odd 20 nm FWHM steps)—cover the near-UV and visible spectrum, with 30 nm FWHM steps for NIR. This discrete, stepwise coverage enables accurate spectral unmixing, ensuring precise identification of multiple fluorescent markers in a single sample.

Key features include ODavg > 5 blocking from 330–900 nm, > 5 OD at laser lines to reduce bleedthrough, and hard-coated quality for spectral repeatability. Compact 12.5 mm housed filters with 10 mm clear aperture are standard, with custom sizes available to fit unique instrument footprints. With high transmission (T > 93%) and a complete 52-filter family, Nanopede™ supports both immediate prototyping and future high-volume production needs.

Backed by IDEX Health & Science expertise, the Nanopede™ family offers precision-engineered optical solutions, OEM-proven reliability, and dedicated support for custom requirements. From flow cytometry to breadboarding, these versatile filters provide a next-generation, value-driven approach to full-spectrum optical detection.

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