Application Note

Empowering Multiplex Polymerase Chain Reaction (PCR)

IDEX - semrock filters

When developing a new multiplex PCR system, whether digital PCR (dPCR) or quantitative PCR (qPCR), Semrock is positioned to assist with optimal optical filter deployment throughout the entire process.

Design Phase: Start with filter selection—Semrock provides a broad range of off-the-shelf filters compatible with common dyes like FAM, SYBRGreen, and Cy5. For more tailored needs, custom OEM designs can be created based on specific requirements. The next step involves analysis using Semrock’s SearchLight fluorescence spectral viewer, which aids in optimizing passband locations to enhance signal-to-background ratios and minimize crosstalk across optical channels. Additionally, Semrock’s experienced application support team collaborates with developers for effective multi-channel system design.

Prototyping: Semrock ensures a quick turnaround from filter design to the shipment of qualified filters in prototype quantities, all at competitive prices. Filters are manufactured with tight tolerances to meet precise dimensional standards, and housing assemblies can be included to streamline in-house workflows.

Production: In the production phase, Semrock guarantees quality filters that meet consistent specifications, supported by highly automated manufacturing processes. Each filter undergoes rigorous quality checks, ensuring excellent lot-to-lot spectral consistency.

By leveraging Semrock's resources—from design tools to manufacturing excellence—developers can enhance the performance and reliability of their multiplex PCR systems, saving time and achieving optimal results. Explore the SearchLight tool at searchlight.idex-hs.com to streamline your workflow further.

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