White Paper: Optimization Of Spinning Disk Confocal Microscopy: Synchronization With The Ultra-Sensitive EMCCD
Source: Andor Technology PLC
The advent of Electron Multiplying Charge Coupled Device (EMCCD) technology and it's ability to overcome previous
hurdles in low-light fluorescence microscopy, such as phototoxicity to live cells, photobleaching of fluorophores and
exposure time restrictions, has resulted in a significant resurgence of interest in use of confocal spinning disk techniques
for live cell microscopy. Here provide an understanding of, and technical solutions to, the issues of synchronization that
have previously marred the coupling of fast CCD camera technology to confocal spinning disk arrangements. We
examine the challenges arising from both old and new models of the Nipkow spinning disk confocal unit and suggest
solutions throughout based on a sound comprehension of both (a) relative scan/exposure times; (b) relative orientation of
the coupled devices; (c) optimisation of EMCCD clocking parameters.
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