Optical Filters For Flow Cytometry

Source: Iridian Spectral Technologies

Optical Filters For Flow Cytometry

Iridian Spectral Technologies designs and manufactures optical filters that are optimized for flow cytometry applications. These dichroic (FLD) and emission (FLE) filters deliver more signals with less background noise, and are compatible with common excitation sources and fluorophores. They also feature high transmission and reflection levels, low ripple, steep edges, and deep blocking outside of the pass band.

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The Iridian FLD and FLE filters are capable of optimizing the discrimination of light emitted from different fluorophores in the optical path of multi-color flow cytometers. These filters eliminate background excitation and the overlap of emission signals from different fluorophores while maintaining high channel signal, leading to improved data capture and analysis.

Dichroic filter specifications include:

  • Tx >90% average; cut-off +/-10 nm
  • Reflection >97%
  • AOI: 45 +/- 2 degrees
  • Ring mounted 25.0 mm OD x 3.5 mm
  • Custom sizing available

Emission filter specifications include:

  • Tx >92% average
  • Average blocking >OD6 from 350 -1000nm
  • AOI: 0 +/- 2 degrees
  • Ring mounted 25.0 mm OD x 5.0 mm
  • Custom sizing available

Download the datasheet for more information on Iridian Spectral Technologies’ optical filters for flow cytometry.

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Iridian Spectral Technologies