Dual Inverted Selective Plane Illumination Microscopy (diSPIM)

Source: Applied Scientific Instrumentation

Dual Inverted Selective Plane Illumination Microscopy (diSPIM)

ASI’s diSPIM (dual inverted selective plane illumination microscopy) system can be mounted to an inverted microscope, or it can be used as a complete system in conjunction with the RAMM platform.

The diSPIM provides several advantages over conventional microscopy systems. It uses conventional mounting/glass cover slips, can generate 3D volumes with isotropic resolution, provides 2x better resolution than confocal or spinning disk systems, achieves a 7-10 fold reduction in photobleaching, and has an acquisition rate of up to 200 images per second (or 2-5 volumes per second) – roughly ten times faster than spinning-disk microscopes.

This system has been used successfully with cells cultured on cover slips and cells embedded in collagen cells, as well as on nematode and zebrafish embryos. For additional information on features and specifications, download the datasheet.