Application Note

Cobolt Flamenco 660 nm for Stimulated Emission Depletion (STED) Microscopy

Source: Cobolt AB

STED (Stimulated Emission Depletion) Microscopy is a technique used to overcome light diffraction and spatial resolution images encountered in traditional optical microscopy. STED Microscopy goes beyond the spatial diffraction-limit, allowing for clear image capture of the fabric of living cells. STED Microscopy, along with GSD (Ground-State Depletion) Microscopy, are also known as fluorescence nanoscopy. This application note presents the results of an experiment conducted through the use of a Cobolt Flamenco™ 660 nm continuous wave single frequency DPSS laser in a STED nanoscopy setup.