Application Note

CCD Cameras And Imaging Spectrographs Facilitate Acquisition Of Femtosecond Stimulated Raman Spectroscopy Data

Source: Princeton Instruments

Characterizing surface-enhanced Raman spectroscopy (SERS) biosensors, or fluorescent dye molecules for in vivo bioanalyte detection, can be difficult due to the overwhelming isoenergetic fluorescence signal that makes it challenging to measure resonance Raman cross-sections for molecules. As a solution, etalon-based femtosecond stimulated Raman spectroscopy (FSRS) has been used to acquire a stimulated Raman signal without strong fluorescence or interference from signals. This article focuses on this approach to determining the resonance Raman cross-sections for use in biological SERS sensing with femtosecond stimulated Raman spectroscopy. Download the full article for more information.