What Is FRET?

Understanding molecular interactions at the nanoscale level is crucial in various scientific fields, from molecular biology to material science. Fluorescence Resonance Energy Transfer (FRET) stands out as a powerful technique in this domain, capable of discerning distance-dependent interactions both in vivo and in vitro. By exploiting the principles of energy transfer between donor and acceptor fluorophores, FRET enables precise measurements of molecular distances. However, its efficacy hinges on meticulous experimental setups, including optimized optical filters and careful consideration of instrumentation, from filter cubes to camera setups.

Join us as we delve deeper into the realm of FRET, where every photon holds a clue to unraveling the mysteries of molecular interactions.