Bringing Fluorescence Images Into Full View With A Novel Hyperspectral Imager

This article explores the use of a SnapShot imager in fluorescence imaging via microscope for the very first time. Utilizing the latest imaging sensor, the Snapshot imager is both compact and attachable via C-mount to any commercially available light microscope. Using this setup, fluorescence hypercubes of several cells were generated, containing both spatial and spectral information.  The fluorescence images were acquired with one shot operation for all the emission range from visible to near infrared (VIS-IR).  The paper will present the hypercubes obtained images from example tissues (475-630nm).  This study demonstrates the potential of application in cell biology or biomedical applications for real time monitoring.