Analysis Of Fluorescent Substances And Binary Mixtures Through Shifted Excitation Raman Difference Spectroscopy

Raman spectroscopy is used to provide both qualitative and quantitative analysis of materials in a wide variety of applications. While the technique has proven to be an effective way to measure substances, there is still an issue with the natural fluorescence many substances possess. This can cause sample contamination with fluorescent compounds in the substance that is being analyzed through Raman spectroscopy. This white paper discusses the implementation of SERDS, or shifted excitation Raman difference spectroscopy, being combined with two wavelength-stabilized laser diodes with fixed wavelength separation in order to deal with fluorescence effects that occur during Raman spectroscopic analysis.