Many modern biological and biomedical research applications rely in part on fluorescence imaging to understand cellular structures and events. This application note details the techniques involved in fluorescence imaging, as well as possible imaging considerations, providing a point of reference for quantitating data obtained from images or movies.
PCO will introduce its latest camera innovation: the pco.panda 4.2 back-illuminated, state-of-the-art sCMOS camera featuring the same compact 65 x 65 x 65 mm dimensions of the renowned pco.panda. PCO's new back-illuminated sensor provides outstanding quantum efficiency, up to 95 percent.
Total internal reflection fluorescence (TIRF) microscopy is used to watch biological processes unfold in real time. Taking advantage of the ability to label individual molecules with different colors of fluorescent tags, TIRF microscopy lets scientists view the complex molecular assemblies that govern cellular processes.
Utilizing interference microscopy techniques to measure the many aspects of surface structures takes advantage of rapid, non-contact operation and rapid data acquisition, often at data acquisition rates in the millions of 3D image points per second.
Nanotechnology uses the concept that constant velocity will get you to the destination faster than constantly stopped traffic. One technique puts this concept into practice via a fast nano-focus device based on a piezo-ceramic actuator embedded in a flexure guided lens positioning mechanism.
Glass-to-metal sealing technology, already used in other harsh-environment applications such as aviation, aerospace, and automotive safety, has emerged as an ideal solution in the development and manufacture of medical connectors.
Datasheets can be misleading, so how do you know what you are really buying? To help optical designers and engineers view optical datasheets more critically and ask the right questions of lens providers, this article discusses key datasheet parameters.
InGaAs pixel chips are utilized in products like 2D NIR/SWIR cameras, NIR/SWIR linear arrays, and avalanche photodiodes. This app note presents the process of creating NIR pixel chips, starting at the crystal growth of semiconducting InGaAs on an InP substrate wafer to the product inspection.