Novel Time-Resolved FLIM Measurements Method
Given the increasing reliance on time-resolved fluorescence lifetime imaging microscopy (TRFLIM) measurements for various biological and clinical investigations, including calcium concentration, protein-protein interaction, cell locomotion, cancer research, and Alzheimer’s disease research, the joint utilization of new picosecond gating technology and a TRFLIM-specific rapid lifetime determination (RLD) processing algorithm can be of great benefit to researchers.
This application note describes the TRFLIM system setup currently being used by Dr. Ammasi Periasamy at the University of Virginia’s W. M. Keck Center for Cellular Imaging. The application note demonstrates how the TRFLIM system, which incorporates the RLD processing algorithm as well as a PI-MAX4: 1024i ICCD camera, has been shown to determine single- and double-component lifetimes very accurately, even when little is known about the system under study.
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