Article
Spectral Imaging For Bioscience Applications: Band-Sequential Techniques
January 20, 2012
What is spectral imaging good for?
Spectral imaging (also referred to as multispectral or hyperspectral imaging) is a technique that has been in research and development in the biosciences for decades now, and is finally gaining some traction as the hardware and software have improved, real application needs are emerging and convenient multispectral reagents, including quantum dots and labeled primary antibodies, are becoming available. What spectral imaging can offer to biology and biomedicine is optimized detection, validation, separation, and quantitation. These can be accomplished through spectral unmixing software that can separate each signal quantitatively into its own channel—without crosstalk—and help eliminate confounding effects such as those due to the presence of unwanted sample autofluorescence.
We will briefly describe a number of spectral imaging technologies, and highlight the advantages and disadvantages of one particular strategy, namely band-sequential image acquisition.
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